will this tubing fit the 40 mil. airstone?

No It will not 40 Mil. Is way to big. That is 1.75 inches round. However 4 mil is a bit under 1/4 inch. A 1/4 inch is equal to about 6 mill I would say that This tube is 6 mil and it fits real tight over the 1/4 inch fitting of the air pump. So much so I had to Chew the end to loosen it, Then shove it on. It works.

I use aquarium tubing to siphon gas out of my snow blower into my lawn mower in the spring time. Then I make sure it is drained and left out in the sun to evaporate out, then I throw it away. My guess is that this tubing should work well for short minutes of durations like that and then make sure it has at least a week chance to dry out before disposing of it. I would never use tubing not rated for gas lines to fix a gas line on a lawn mower or anything like that.

if my air pump fails, will all the water back-flow and flood the room?

If your pump fails in a very unusual manner, such that both check valves fail, and if it continues to pump with the outlet check valve actually functioning backwards, then it is remotely possible that the water could backflow into the room. But... none of that happens in real life; it only happens in comic books. If your pump fails, it will just stop pumping but both check valves will remain intact so the air line will continue to hold some small amount of pressure until you notice that all your fish are dead and your tank is sans bubbles. In order to start a siphon, which might drain your tank, there would need to be a fair quantity of water in the tubing OUTSIDE your tank, the weight of which will draw suction. What's more, that fair quantity of external water needs to be BELOW the (top) surface of the water in your tank. So... for all intents and purposes... no. It's not going to happen.

will this tubing fit on 4mm(air stone) and 5mm(check valve) outer diameter connections?

It will not. It might fit on 3.5mm fittings, because that's the inside diameter of this tubing.

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🌟 Keep your aquarium breathing easy with Penn-Plax’s ultimate airline tubing!

FLEX WITHOUT THE FUSS - Engineered to resist kinks, maintaining consistent air delivery without interruptions.
LIGHTWEIGHT DURABLE - At just 110 grams, it’s easy to handle yet built to last through countless aquatic adventures.
FISH FRIENDLY MATERIAL - Crafted from safe, non-toxic plastic ideal for both freshwater and saltwater environments.
UNIVERSAL COMPATIBILITY - Designed to fit standard air pumps and accessories, making upgrades effortless.
ENDLESS AQUARIUM CONNECTIVITY - 25 feet of premium airline tubing ensures seamless air flow for your aquatic setup.

Penn-Plax Standard Airline Tubing offers 25 feet of clear, flexible, and kink-resistant tubing designed for reliable air flow in both fresh and saltwater aquariums. Lightweight and made from safe plastic, it ensures compatibility with standard air pumps, making it an essential upgrade for any serious aquarium enthusiast.

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Brand	Penn-Plax
Model Number	ST25
Product Dimensions	25.4 x 1.27 x 7.62 cm; 110 g
Name List for Name Plates	Simple
Material	Plastic
Are Batteries Included	No
Item Weight	110 g

PENN PLAX Standard Airline Tubing Air Pump Accessories, 25-Feet by Penn-Plax

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